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Image Search Results
Journal: Cerebral cortex (New York, N.Y. : 1991)
Article Title: BDNF/MAPK/ERK-induced BMP7 expression in the developing cerebral cortex induces premature radial glia differentiation and impairs neuronal migration.
doi: 10.1093/cercor/bhp275
Figure Lengend Snippet: Figure 2. BDNF upregulates BMP7 in vitro. (A) Quantification of BMP7 mRNA levels by RT-PCR in primary neuronal E16 cortical cultures treated with BDNF (100 ng/mL) for 1 and 6 h. (B) Western blot for BMP7 and actin as loading control in neuronal cultures treated for 6 h with BNDF (100 ng/mL). F9 cells (embryonic carcinoma cells that overexpress BMP7) were used as a positive control. The graph summarizing quantification of western blots demonstrates that neurons treated with BDNF for 6 h express higher BMP7 protein levels. (C) BMP7 mRNA was quantified in pure neonatal glial cultures treated with BDNF (100 ng/mL) for 1 or 6 h. BMP7 mRNA levels increased after 6 h of BDNF treatment in neuronal cultures (A) but not in glial cultures (C).
Article Snippet:
Techniques: In Vitro, Reverse Transcription Polymerase Chain Reaction, Western Blot, Control, Positive Control